Composite

Part:BBa_K4695120:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-03)


dcx1-docxylY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part consists of:

  • Dcx1 coding sequence (BBa_K4695020)
  • DT4_TEV linker (BBa_J435395)
  • DocXylY coding sequence (BBa_K4695040)
  • C-terminal T5E_6H HisTag linker (BBa_J435369)


Sequence is designed in a way that allows user to swap enzymatic and dockerin domains with their counterparts.
FLANKED 5' fusion site: AATG, 3' fusion site: GCTT.
Source sequences were optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/


Source

Dcx1:
Organism: Pantoea dispersa BJQ0007
Article: https://doi.org/10.1016/j.jfca.2021.104201

DocXylY:
Organism: Acetovibrio Thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Registry of standard biological parts

References